P REPARATION OF A NEW MEDIUM FOR IN VITRO CULTURE OF BORRELIA MICROTTI AND BORRELIA PERSICA
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Abstract:
Borrelia microtti and Borrelia persica are two Iranian strains of spirochetes whose vectors are Ornithodorous tholozani and Ornithodorous erraticus which are responsible for relapsing fever. BSK medium has been used for in vitro culture of other strains of borrelia, although the two mentioned strains could not be successfully cultured in it. We have modified BSK medium by using guinea pig and fetal calf serum instead of rabbit serum. It was observed that for in vitro culture of Borrelia persica, guinea pig serum and for Borrelia microtti, fetal calf serum show the best growth.
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Journal title
volume 15 issue 4
pages 227- 230
publication date 2002-02
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